Methods of plant analysis
Properties of all plant organisms and internal structures inherent in individual species are determined by the multifaceted, constantly changing environmental impact. The influence of such factors as climate, soil, as well as the cycle of substances and energy, is significant. Traditionally, to determine the properties of medicinal products or food products, the shares of substances that can be isolated analytically are determined. But these individual substances can not cover all the internal properties, for example, medicinal and spicy aromatic plants. Therefore, such descriptions of individual properties of plants can not satisfy all our needs. The exhaustive description of the properties of herbal medicines, including biological activity, requires a comprehensive, comprehensive study. There are a number of methods that allow to reveal the quality and quantity of biologically active substances in the plant composition, as well as the places of their accumulation.
The luminescent microscopic analysis of
is based on the fact that the biologically active substances contained in the plant give a brightly colored glow in the luminescent microscope, with different chemical substances characterized by different colors. So, alkaloids give a yellow color, and glycosides - orange. This method is used mainly to identify places of accumulation of active substances in plant tissues, and the intensity of luminescence indicates a greater or lesser concentration of these substances. Phytochemical analysis of is designed to identify the qualitative and quantitative index of the content of active substances in the eusthenia. To determine the quality, chemical reactions are used. The amount of active substances in the plant is the main indicator of its good quality, therefore, their volumetric analysis is also carried out using chemical methods. For the study of plants containing active substances such as alkaloids, coumarins,glavones, which require not simple summary analysis, but also their separation into components, chromatographic analysis is performed. Chromatographic analysis method was first introduced in 1903 by the botanist
by Color, and since then various variants have been developed that have a separate
.This method of separating the zeolite mixture into components is based on the difference in their physical and chemical properties. The photographic method, with the help of panchromatic chromatography, can make the internal structure of the plant visible, see the lines, shapes and colors of the plant. Such paintings, obtained from water extracts, are retained on silver-nitrate filter paper and reproduced. The method of interpretation of chromatograms is developing successfully. This technique is supported by data obtained with the help of other, already well-known methods.
Based on the circulation chromatograms, a panoramic chromatography method is being developed to determine the quality of the plant by the presence of nutrients concentrated in it. The results obtained using this method should be supported by data on the analysis of the acidity level of the plant, the interaction of enzymes contained in its composition, etc. The main task of the further development of the chromatographic method of plant analysis should be the search for ways of influencing plant raw materials during its cultivation,, warehousing and at the stage of direct preparation of dosage forms in order to increase the content of valuable active substances in it.